Wastewater RNA-seq is dominated by bacterial rRNA where pathogenic viruses represent just 0.05% of total reads. MTP cuts through the background so you can actually see them.
Click any segment or legend row to explore the internal breakdown of each RNA fraction. The target signal is sub-pixel at this scale, that's the problem.
Proportional chart — pathogenic viruses are sub-pixel at 0.05%
Wastewater is one of the most complex biological matrices in existence. Every sample is a war between signal and noise.
Bacterial rRNA can exceed 80% of all reads while your target viruses sit at 0.05%. Standard RNA-seq wastes 99.95% of your sequencing budget on noise you don't need.
Most pathogenic RNA viruses like norovirus, SARS-CoV-2, and influenza are not polyadenylated or have non-standard poly(A) tails. Standard mRNA enrichment misses them entirely.
The microbial community in wastewater shifts with season, geography, and diet. A fixed panel designed for one catchment may perform poorly in another without recalibration.
A single wastewater sample contains thousands of organisms including bacteria, viruses, fungi, archaea, human cells, and plant material that are all competing for sequencing reads.
Wastewater is a harsh environment. RNA degrades rapidly from RNases, UV exposure, and pH extremes. Enrichment strategies must work on fragmented, low-quality input.
Fixed commercial panels can't anticipate novel variants or emerging pathogens. Surveillance programs need a flexible enrichment strategy that can be updated as threats evolve.
Multi-Targeted Priming is a drop-in primer replacement for reverse transcription. MTP simultaneously enriches your targets and suppresses your off-targets in a single RT step.
Multi-targeted primers are designed to capture any and every pathogenic virus transcript you care about: norovirus, SARS-CoV-2, influenza, adenovirus, and more, regardless of poly(A) status.
Off-target controls for bacterial rRNA, human rRNA, and other dominant background species are built directly into the primer pool. Simply throw MTP at it or combine with existing depletion methods.
When a new variant emerges or your surveillance scope expands, your MTP pool gets updated. No new instruments, no workflow revalidation, just a fresh MTP.
We'll design a custom MTP for your exact surveillance targets: pathogenic viruses, AMR genes, or any combination, with built-in off-targeting of the dominant background fractions specific to your catchment.
Custom design · Any target set · Drop-in RT replacement · No new instruments