Frequently asked questions
What do MultiTarga primers target?
MultiTarga primers target your organism's coding regions, while specifically avoiding the non-coding regions. For RNA-seq this means we target the mRNA and avoid the abundant rRNA and tRNA.
What protocols do MultiTarga primers work with?
Essentially anywhere you use primers, you can drop MultiTarga directly in. First developed for targeted gene amplification in gene expression analysis (GEA), there is now abundant evidence of MultiTarga's efficacy as a replacement for polyA and random hexamer amplification methods. Additionally, we are constantly investigating possible applications of our powerful and versatile amplification method.
Which organisms do MultiTarga primers work on?
MultiTarga can be used with any genome of interest. The patented MultiTarga algorithm simply requires an assembly genome with annotated genes. Our catalog is made up from RefSeq, and we can also support GenBank annotated assemblies as well as any genome file(s) with annotated genes.
How do MultiTarga primers work?
The technology responsible for designing our primers uses an evolutionary algorithm rooted in principled biochemistry and evolutionary biology research. The output of our approach is an optimized set of multi-targeted degenerate probes validated by their fit with the targets and avoidance of the off-targets.
Does MultiTarga collaborate with research partners and consortias?
Absolutely, we are open to collaboration and are currently working with individual labs/researchers, entire sequencing centers, and biotech company partners. We are always looking to improve our methods and expand the applicability of our products; we encourage collaboration .
How do I use MultiTarga?
Simply find your genome of interest, order your MultiTarga Primer, and recieve an experiment-ready primer in the next few days.
Precision Amplification
Inspired by evolutionary biology research and powered by machine learning our versatile primer design method delivers precision genomics to any organism.
Protocol Flexibility
MultiTarga primers work in any sequencing experiment that requires amplification or capture of specific targets. Add MultiTarga into your Library Prep protocols and keep your existing workflow all while getting better results.
Organismal Reach
Our primer design method works for genetic material from any organism. Need to amplify one organism's genetic material in a parasite host system? Do you have a unique metagenomics problem? Contact us with your special request to apply MultiTarga to any amplicon set.