Sequence with Power and Precision.

Species-level Specificity

Precision targeting for your exact research needs with unparalleled accuracy.

Genome-wide Scalability

Seamlessly scale from targeted genes to comprehensive genome-wide analysis.

Protocol Flexibility

Adaptable to your existing workflows with no new hardware requirements.

The Multi-Targeted Primer

(MTP)

has Arrived.

Scalable selective enrichment is here, and it's as easy as swapping in MTP for Oligo(dT).

The RNA Revolution

We are in the era of RNA. It's no longer just a messenger; it's a master regulator of life. But as our understanding deepens, we need smarter tools to decipher its complexity, moving beyond the limitations of outdated methods like poly(A) enrichment.

Introducing: The Multi-Targeted Primer (MTP)

MTP is your solution for specific, scalable, and flexible selective enrichment.

The Enrichment Pipeline

MultiTarga leverages big data bioinformatics, graph representation learning, and principled evolutionary biology to create MTPs.

Adaptable Discriminative Selection

Precisely differentiates target RNA sequences from off-target noise, one tissue from another, or one organism from a multi-organismal sample.

Core Advantages

Species-level Specificity

Precision targeting for your exact research needs with unparalleled accuracy.

Focus your telescope on the RNA species you care about, eliminating irrelevant signals.

Genome-wide Scalability

Seamlessly scale from targeted genes to comprehensive genome-wide analysis.

Efficiently analyze everything from specific transcripts to the entire transcriptome.

Protocol Flexibility

Adaptable to your existing workflows with no new hardware requirements.

Effortless integration into reverse transcription, bead-capture, and single cell library construction workflows.

Versatile Applications: How MTP Works for You

Its nature as a simple, yet powerful, primer makes it incredibly versatile:

Targeted cDNA Synthesis

Reverse transcription that is truly representative of what you care about.

Hybridization Bead-Capture / Depletion

Isolate those hard to find RNA species with precision.

scRNA-seq Compatability

10x protocol spike-in, sciRNA-seq integration, post-enrichment on prepared libraries.

Adaptable Workflows

No new equipment required to improve detection of low abundance genes and differential expression.

Compatible Library Preparation Kits

For RNA-seq library preparation that includes a first-strand cDNA synthesis step, MultiTarga is a simple 1-step swap-in. Below are compatible kits:

Illumina

TruSeq Stranded mRNA

Supports upstream RT prior to library prep

Cat. No. 20020594

TruSeq Stranded Total RNA with Ribo-Zero

Supports upstream RT prior to library prep

Cat. No. 20020596

RNA Prep with Enrichment

Compatible with user-prepared cDNA workflows

Cat. No. 20040536

AmpliSeq for Illumina Custom RNA Panel

RT step separable; Randomer substitution for MTPs

Cat. No. 20020496

Thermo Fisher

SuperScript IV First-Strand Synthesis System

Targeted primers allowed for RT

Cat. No. 18091050

Maxima H Minus First Strand cDNA Synthesis Kit

Targeted primers allowed for RT

Cat. No. K1651

NEB

ProtoScript II First Strand cDNA Synthesis Kit

Designed for targeted primer usage

Cat. No. E6560

NEBNext Ultra II RNA Library Prep

Accepts externally Prepared cDNA

Cat. No. E7770

NEBNext Ultra II Directional RNA Library Prep

Accepts externally Prepared cDNA

Cat. No. E7760

Don't see your kit listed? Contact us at contact@multitarga.com - MTP will most likely work for your preferred kit and we are excited to validate all new workflow integrations.

The MultiTarga Leap: Beyond Conventional Priming

Move past the constraints of 1970's reverse transcription. MTP offers:

Superior Data

Reduce sequencing waste, enhance sensitivity for low-abundance or non-polyadenylated RNAs often missed by Oligo(dT).

Greater Control

Achieve more specific, reliable, and representative results than with random priming.

Step into the Future with MTP.

Experience the power of specificity, scalability, and adaptability. Welcome to MultiTarga.